您当前的位置: 首页 >> 科研成果 >> 正文

【论文发表】Triterpenoids from Ganoderma gibbosum: A Class of Sensitizers of FLC-Resistant Candida albicans to Fluconazole

2019年08月23日 16:26  点击:[]

Triterpenoids from Ganoderma gibbosum: A Class of Sensitizers of FLC-Resistant Candida albicans to Fluconazole

By:Pu, Debing;Li, Xiaoning;Lin, Jing;Zhang, Ruihan;Luo, Ting;Wang, Yuan;Gao, Junbo;Zeb, Muhammad Aurang;Zhang, Xingjie;Li, Xiaoli;Wang, Ruirui;Xiao, Weilie

Journal of natural products

Volume:82

Issue:8

Pages:2067-2077

DOI:10.1021/acs.jnatprod.9b00148


Abstract


Fungal drug resistance is a major health threat, and reports of clinical resistance worldwide are becoming increasingly common. In a research program to discover new molecules to help overcome this problem, 14 new lanostane-type triterpenoids, gibbosicolids A-G (2-8) and gibbosic acids I-O (9-15), were isolated from the fruiting bodies of Ganoderma gibbosum, along with seven known triterpenoid derivatives. These compounds featured high levels of oxidation, epimerization, and gamma-lactonization. Structures were elucidated by comprehensive spectroscopic analyses and HRMS data. Absolute configurations were assigned based on quantum chemical calculations, including calculated chemical shift with DP4+ analysis, coupling constants, and electronic circular dichroism (ECD) methods. Results show that the calculated NMR with DP4+ analysis could not reliably establish the overall spatial configuration of molecules possessing independent and free-rotational stereoclusters. All these compounds significantly increased the sensitivity of fluconazole (FLC)-resistant C. albicans to FLC. Compounds 2, 5, 9, 12, 16, 17, and 21 exhibited strong antifungal activity against FLC-resistant C. albicans when combined with FLC, with MIC50 values ranging from 3.8 to 8.8 mug/mL.


上一条:【论文发表】Retro-biosynthetic construction of corynanthe alkaloid skeletons from rhynchophylline alkaloids 下一条:【论文发表】A stable silver metallacage with solvatochromic and mechanochromic behavior for white LED fabrication

关闭

联系我们
  • 电话:0871-65031119
  • 地址:昆明市呈贡区大学城东外环南路